pAAV_Efla_FLEX_APEX2-eGFPf
(Plasmid
#171939)
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PurposeExpresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171939 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAPEX2-eGFPf
- Promoter Ef1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer aggccagcttggcacttga (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_Efla_FLEX_APEX2-eGFPf was a gift from David Berson (Addgene plasmid # 171939 ; http://n2t.net/addgene:171939 ; RRID:Addgene_171939)
