pJK2020
(Plasmid
#171932)
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Purposeexpress MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171932 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCFJ151
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
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SpeciesC. elegans (nematode)
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Insert Size (bp)3563
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GenBank ID31378588
- Promoter -
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Tag
/ Fusion Protein
- superfolder GFP, mCherry
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK2020 was a gift from Judith Kimble (Addgene plasmid # 171932 ; http://n2t.net/addgene:171932 ; RRID:Addgene_171932) -
For your References section:
Dynamics of Notch-Dependent Transcriptional Bursting in Its Native Context. Lee C, Shin H, Kimble J. Dev Cell. 2019 Aug 19;50(4):426-435.e4. doi: 10.1016/j.devcel.2019.07.001. Epub 2019 Aug 1. 10.1016/j.devcel.2019.07.001 PubMed 31378588
