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Addgene

pET-28c S2
(Plasmid #171920)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171920 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28c
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5213
  • Total vector size (bp) 5349
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNA-based (p)ppGpp sensor
  • Species
    Synthetic
  • Insert Size (bp)
    136
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28c S2 was a gift from Mingxu You (Addgene plasmid # 171920 ; http://n2t.net/addgene:171920 ; RRID:Addgene_171920)

  • For your References section:

    Live-Cell Imaging of Guanosine Tetra- and Pentaphosphate (p)ppGpp with RNA-based Fluorescent Sensors*. Sun Z, Wu R, Zhao B, Zeinert R, Chien P, You M. Angew Chem Int Ed Engl. 2021 Nov 2;60(45):24070-24074. doi: 10.1002/anie.202111170. Epub 2021 Oct 5. 10.1002/anie.202111170 PubMed 34487413
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