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Addgene

M36 Dpr C-terminal GST
(Plasmid #17192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    XDpr
  • Alt name
    Dapper
  • Alt name
    dact1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1200
  • Entrez Gene
    dact1-b (a.k.a. dpr, dpr1, dact1, frodo, hdpr1, Dapper, thyex3, dapper1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Coding sequence of original XDpr prey clone (M35) cloned into pGEX vector as a 1.2 kb EcoR1-Xba1 fragment (Xba site is DAM methylated).

Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M36 Dpr C-terminal GST was a gift from Randall Moon (Addgene plasmid # 17192 ; http://n2t.net/addgene:17192 ; RRID:Addgene_17192)

  • For your References section:

    Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Cheyette BN, Waxman JS, Miller JR, Takemaru K, Sheldahl LC, Khlebtsova N, Fox EP, Earnest T, Moon RT. Dev Cell. 2002 Apr. 2(4):449-61. 10.1016/S1534-5807(02)00140-5 PubMed 11970895
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