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M35 Dpr yeast clone
(Plasmid #17191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGAD
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    XDpr
  • Alt name
    Dapper
  • Alt name
    Dpr
  • Alt name
    Dact1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1600
  • Entrez Gene
    dact1-b (a.k.a. dpr, dpr1, dact1, frodo, hdpr1, Dapper, thyex3, dapper1)
  • Tag / Fusion Protein
    • Gal4-AD (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Dpr yeast clone in original prey vector. 1.6kb EcoR1 fragment corresponds to C-terminal 1.2 kb of Xenopus Dpr and 3' UTR in pGAD vector.

Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M35 Dpr yeast clone was a gift from Randall Moon (Addgene plasmid # 17191 ; http://n2t.net/addgene:17191 ; RRID:Addgene_17191)

  • For your References section:

    Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Cheyette BN, Waxman JS, Miller JR, Takemaru K, Sheldahl LC, Khlebtsova N, Fox EP, Earnest T, Moon RT. Dev Cell. 2002 Apr. 2(4):449-61. 10.1016/S1534-5807(02)00140-5 PubMed 11970895
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