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M34 Xdsh PDZ bait-pGBT9
(Plasmid #17190)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17190 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBT9
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Xdsh
  • Alt name
    Dishevelled
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    800
  • Mutation
    Dsh bp 343-1191
  • Entrez Gene
    dvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
  • Tag / Fusion Protein
    • Gal4-DBD (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer Gal4-N-term-primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: 0.8 kb EcoR1-BamHI Xdsh cDNA (bp 343-1191 of Dsh), including the basic and PDZ regions, subcloned into yeast two hybrid bait vector GBT9.

Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M34 Xdsh PDZ bait-pGBT9 was a gift from Randall Moon (Addgene plasmid # 17190 ; http://n2t.net/addgene:17190 ; RRID:Addgene_17190)

  • For your References section:

    Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Cheyette BN, Waxman JS, Miller JR, Takemaru K, Sheldahl LC, Khlebtsova N, Fox EP, Earnest T, Moon RT. Dev Cell. 2002 Apr. 2(4):449-61. 10.1016/S1534-5807(02)00140-5 PubMed 11970895
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