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Addgene

pGEX-GST-His6-1_HBD
(Plasmid #171869)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PGEX-2TK
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    T7 Express lysY/Iq (NEB #C3013) should be used for expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hybrid binding domain of Rnase H1
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Tag / Fusion Protein
    • GST- and His10-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-GST-His6-1_HBD was a gift from Kaiwei Liang (Addgene plasmid # 171869 ; http://n2t.net/addgene:171869 ; RRID:Addgene_171869)

  • For your References section:

    Genomic profiling of native R loops with a DNA-RNA hybrid recognition sensor. Wang K, Wang H, Li C, Yin Z, Xiao R, Li Q, Xiang Y, Wang W, Huang J, Chen L, Fang P, Liang K. Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe3516. doi: 10.1126/sciadv.abe3516. Print 2021 Feb. 10.1126/sciadv.abe3516 PubMed 33597247
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