pBI/Rabaptin-5
(Plasmid
#171851)
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Purposeinducible expression of Rabaptin-5 in mammalian cells via co-transfection with pTet-Off.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171851 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBI
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Backbone manufacturerTakara Biotech/Clontech
- Backbone size w/o insert (bp) 4358
- Total vector size (bp) 6947
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRabaptin-5 (human)
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SpeciesH. sapiens (human)
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Insert Size (bp)2589
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GenBank IDNP_004694.2
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Marino Zerial's group at MPI-CBG in Germany
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBI/Rabaptin-5 was a gift from Guangpu Li (Addgene plasmid # 171851 ; http://n2t.net/addgene:171851 ; RRID:Addgene_171851) -
For your References section:
Delayed onset of positive feedback activation of Rab5 by Rabex-5 and Rabaptin-5 in endocytosis. Zhu H, Qian H, Li G. PLoS One. 2010 Feb 16;5(2):e9226. doi: 10.1371/journal.pone.0009226. 10.1371/journal.pone.0009226 PubMed 20169068