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pcDNA3-FLAG_FBXL7 (R310H)
(Plasmid #171849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171849 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6870
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    F-box and leucine rich repeat protein 7
  • Alt name
    FBXL7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1470
  • Mutation
    changed Arginine 310 to Histidine
  • GenBank ID
    NM_012304.5
  • Entrez Gene
    FBXL7 (a.k.a. FBL6, FBL7)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FLAG_FBXL7 (R310H) was a gift from Michele Pagano (Addgene plasmid # 171849 ; http://n2t.net/addgene:171849 ; RRID:Addgene_171849)

  • For your References section:

    Epigenetic silencing of the ubiquitin ligase subunit FBXL7 impairs c-SRC degradation and promotes epithelial-to-mesenchymal transition and metastasis. Moro L, Simoneschi D, Kurz E, Arbini AA, Jang S, Guaragnella N, Giannattasio S, Wang W, Chen YA, Pires G, Dang A, Hernandez E, Kapur P, Mishra A, Tsirigos A, Miller G, Hsieh JT, Pagano M. Nat Cell Biol. 2020 Sep;22(9):1130-1142. doi: 10.1038/s41556-020-0560-6. Epub 2020 Aug 24. 10.1038/s41556-020-0560-6 PubMed 32839549
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