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Addgene

pJK2067
(Plasmid #171845)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171845 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ151
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    his-58, eGFP, mCherry
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    4215
  • Mutation
    boxB wild type and hairpin mutant
  • GenBank ID
    - -
  • Promoter mex-5 promoter
  • Tag / Fusion Protein
    • N-terminal Tag OLLAS, V5 and C-terminal Tag PEST domain

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK2067 was a gift from Judith Kimble (Addgene plasmid # 171845 ; http://n2t.net/addgene:171845 ; RRID:Addgene_171845)

  • For your References section:

    C. elegans germ granules require both assembly and localized regulators for mRNA repression. Aoki ST, Lynch TR, Crittenden SL, Bingman CA, Wickens M, Kimble J. Nat Commun. 2021 Feb 12;12(1):996. doi: 10.1038/s41467-021-21278-1. 10.1038/s41467-021-21278-1 PubMed 33579952
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