tdTomato-anti-AlfaTag nanobody
(Plasmid
#171819)
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PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobody
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepColdII
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Backbone manufacturerTakara
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametdTomato-anti-AlfaTag nanobody
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SpeciesSynthetic
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Tags
/ Fusion Proteins
- 6xHis (N terminal on insert)
- SEP tag (6xD) (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tdTomato-anti-AlfaTag nanobody was a gift from Ikuo Wada (Addgene plasmid # 171819 ; http://n2t.net/addgene:171819 ; RRID:Addgene_171819) -
For your References section:
Phosphorylation of human phospholipase A1 DDHD1 at newly identified phosphosites affects its subcellular localization. Matsumoto N, Nemoto-Sasaki Y, Oka S, Arai S, Wada I, Yamashita A. J Biol Chem. 2021 Jul;297(1):100851. doi: 10.1016/j.jbc.2021.100851. Epub 2021 Jun 3. 10.1016/j.jbc.2021.100851 PubMed 34089703