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Addgene

M29 NacMS+LEF>Nac
(Plasmid #17179)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17179 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    n/a

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nacre promoter driving nacre cDNA
  • Alt name
    nacre
  • Species
    D. rerio (zebrafish)
  • Mutation
    All three Tcf/Lef sites are mutated, but 5 LEF sites are added at a new position
  • GenBank ID
    AF211890
  • Entrez Gene
    mitfa (a.k.a. nacre, z3A.1)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note for Use:

Description: The NacMS>Nac construct (with mutations in endogenous.
LEF sites) was engineered to have 5 LEF sites added at a new position to.
"rescue" beta-catenin responsiveness of the promoter.

Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M29 NacMS+LEF>Nac was a gift from Randall Moon (Addgene plasmid # 17179 ; http://n2t.net/addgene:17179 ; RRID:Addgene_17179)

  • For your References section:

    Direct regulation of nacre, a zebrafish MITF homolog required for pigment cell formation, by the Wnt pathway. Dorsky RI, Raible DW, Moon RT. Genes Dev. 2000 Jan 15;14(2):158-62. PubMed 10652270
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