pET28-MBP-super TEV protease
(Plasmid #171782)

• Purpose: Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
• Depositing Lab: Mark Howarth
• Publication: Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 10.1016/j.chembiol.2021.07.005.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7295
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MBP-sTEV
• Species: Synthetic
• Insert Size (bp): 1926
• Mutation: No internal TEV cleavage site between the MBP and TEV protease. The TEV protease domain contains the following solubility/stability mutations (numbers refer to the standard TEV protease numbering scheme): C19V L56V C110V C130S S135G and S219D
• Promoter: T7
• Tags / Fusion Proteins:
  • Internal His6 tag
  • Arg6 tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry