pET28-sfGFP-SpyTag003 Loop A
(Plasmid
#171776)
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PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171776 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6203
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP-SpyTag003 Loop A
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SpeciesSynthetic
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Insert Size (bp)834
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MutationSpyTag003 and linkers inserted between residues Val22 and Asn23
- Promoter T7
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Tag
/ Fusion Protein
- His6 (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-sfGFP-SpyTag003 Loop A was a gift from Mark Howarth (Addgene plasmid # 171776 ; http://n2t.net/addgene:171776 ; RRID:Addgene_171776) -
For your References section:
DogCatcher allows loop-friendly protein-protein ligation. Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang J, Bon RS, Howarth M. Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 10.1016/j.chembiol.2021.07.005. 10.1016/j.chembiol.2021.07.005 PubMed 34324879
