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M27 NacMS>luc
(Plasmid #17177)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nacre promoter
  • Alt name
    nacre
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    800
  • Mutation
    All three Tcf/Lef sites are mutated
  • GenBank ID
    AF211890
  • Entrez Gene
    mitfa (a.k.a. nacre, z3A.1)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: zebrafish nacre promoter with all three LEF sites mutated, driving luciferase reporter. This is a control construct.

Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M27 NacMS>luc was a gift from Randall Moon (Addgene plasmid # 17177 ; http://n2t.net/addgene:17177 ; RRID:Addgene_17177)

  • For your References section:

    Direct regulation of nacre, a zebrafish MITF homolog required for pigment cell formation, by the Wnt pathway. Dorsky RI, Raible DW, Moon RT. Genes Dev. 2000 Jan 15;14(2):158-62. PubMed 10652270
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