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pLIC-His-GBAi W211A
(Plasmid #171756)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171756 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIC-His
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GBAi
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    960
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIC-His-GBAi W211A was a gift from Mikel Garcia-Marcos (Addgene plasmid # 171756 ; http://n2t.net/addgene:171756 ; RRID:Addgene_171756)

  • For your References section:

    Specific inhibition of GPCR-independent G protein signaling by a rationally engineered protein. Leyme A, Marivin A, Maziarz M, DiGiacomo V, Papakonstantinou MP, Patel PP, Blanco-Canosa JB, Walawalkar IA, Rodriguez-Davila G, Dominguez I, Garcia-Marcos M. Proc Natl Acad Sci U S A. 2017 Nov 28;114(48):E10319-E10328. doi: 10.1073/pnas.1707992114. Epub 2017 Nov 13. 10.1073/pnas.1707992114 PubMed 29133411
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