pLIC-myc-GBAi W211A
(Plasmid
#171754)
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PurposeFor the mammalian expression of myc-GBAi W211A
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLIC-myc
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGBAi
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SpeciesR. norvegicus (rat), Synthetic
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Insert Size (bp)960
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLIC-myc-GBAi W211A was a gift from Mikel Garcia-Marcos (Addgene plasmid # 171754 ; http://n2t.net/addgene:171754 ; RRID:Addgene_171754) -
For your References section:
Specific inhibition of GPCR-independent G protein signaling by a rationally engineered protein. Leyme A, Marivin A, Maziarz M, DiGiacomo V, Papakonstantinou MP, Patel PP, Blanco-Canosa JB, Walawalkar IA, Rodriguez-Davila G, Dominguez I, Garcia-Marcos M. Proc Natl Acad Sci U S A. 2017 Nov 28;114(48):E10319-E10328. doi: 10.1073/pnas.1707992114. Epub 2017 Nov 13. 10.1073/pnas.1707992114 PubMed 29133411
