M25 NacMS>Nac
(Plasmid
#17175)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17175 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonen/a
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namenacre promoter driving nacre cDNA
-
Alt namenacre
-
SpeciesD. rerio (zebrafish)
-
MutationAll three Tcf/Lef sites are mutated
-
GenBank IDAF211890
-
Entrez Genemitfa (a.k.a. nacre, z3A.1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note for Use:
Description: zebrafish nacre promoter driving nacre cDNA. All three LEF.
sites are mutated, so this is a control construct.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
M25 NacMS>Nac was a gift from Randall Moon (Addgene plasmid # 17175 ; http://n2t.net/addgene:17175 ; RRID:Addgene_17175) -
For your References section:
Direct regulation of nacre, a zebrafish MITF homolog required for pigment cell formation, by the Wnt pathway. Dorsky RI, Raible DW, Moon RT. Genes Dev. 2000 Jan 15;14(2):158-62. PubMed 10652270
Map uploaded by the depositor.
