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E2_pENTR_R4R3_T CTP
(Plasmid #171736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171736 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PINII
  • Vector type
    Multisite Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
  • Species
    Synthetic

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Multisite Gateway entry vector to terminate E1 ORF and having RPS5A promoter with RecA1 plastid targeting signal for E3 ORF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E2_pENTR_R4R3_T CTP was a gift from Shin-ichi Arimura (Addgene plasmid # 171736 ; http://n2t.net/addgene:171736 ; RRID:Addgene_171736)

  • For your References section:

    Targeted base editing in the plastid genome of Arabidopsis thaliana. Nakazato I, Okuno M, Yamamoto H, Tamura Y, Itoh T, Shikanai T, Takanashi H, Tsutsumi N, Arimura SI. Nat Plants. 2021 Jul;7(7):906-913. doi: 10.1038/s41477-021-00954-6. Epub 2021 Jul 1. 10.1038/s41477-021-00954-6 PubMed 34211131
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