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pDest_pK7WG2_pRPS5A_CTP OleGFP
(Plasmid #171723)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171723 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pK7WG2
  • Backbone manufacturer
    VIB, Ghent Univ, Kamiri et al
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
  • Species
    Synthetic

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gateway destination vector for plants to express ORFs from entry clone with RecA1 plastid targeting signal under RPS5A promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest_pK7WG2_pRPS5A_CTP OleGFP was a gift from Shin-ichi Arimura (Addgene plasmid # 171723 ; http://n2t.net/addgene:171723 ; RRID:Addgene_171723)

  • For your References section:

    Targeted base editing in the plastid genome of Arabidopsis thaliana. Nakazato I, Okuno M, Yamamoto H, Tamura Y, Itoh T, Shikanai T, Takanashi H, Tsutsumi N, Arimura SI. Nat Plants. 2021 Jul;7(7):906-913. doi: 10.1038/s41477-021-00954-6. Epub 2021 Jul 1. 10.1038/s41477-021-00954-6 PubMed 34211131
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