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Str-KDEL_TNF-SBP-RpHLuorin2
(Plasmid #171722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESneo3
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    RUSH TNFα-RpHLuorin2 was generated by replacing EGFP from Str-KDEL_TNF-SBP-EGFP (Addgene plasmid #65278) with synthetic RpHLuorin2 (Genscript) with restriction sites SbfI and BsrGI.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNF
  • Alt name
    DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_000594.3
  • Entrez Gene
    TNF (a.k.a. DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F)
  • Promoter CMV
  • Tags / Fusion Proteins
    • ratiometric pHluorin2 (C terminal on insert)
    • Streptavidin Binding Protein (SBP) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (unknown if destroyed)
  • 3′ cloning site BsrGI (unknown if destroyed)
  • 5′ sequencing primer IRES-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    TNFα-RpHLuorin2 was generated by replacing EGFP from Str-KDEL_TNF-SBP-EGFP (Addgene plasmid #65278) with synthetic RpHLuorin2 (Genscript)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Str-KDEL_TNF-SBP-RpHLuorin2 was a gift from Geert van den Bogaart (Addgene plasmid # 171722 ; http://n2t.net/addgene:171722 ; RRID:Addgene_171722)

  • For your References section:

    Fluorescence Lifetime Imaging of pH along the Secretory Pathway. Linders PTA, Ioannidis M, Ter Beest M, van den Bogaart G. ACS Chem Biol. 2022 Jan 21;17(1):240-251. doi: 10.1021/acschembio.1c00907. Epub 2022 Jan 10. 10.1021/acschembio.1c00907 PubMed 35000377
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