pBIOS1458
(Plasmid
#171715)
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Purposeexpresses maize Glutamine Synthetase ZmGln1-3 cDNA under the control of the Cassava Vein Mosaic Virus promoter (CsVMV) (cassette 1) and the maize Rubisco small subunit promoter (ZmRbcS) (cassette 2)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171715 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBSII SK+
- Total vector size (bp) 8192
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZmGln1-3
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Alt nameGlutamine Synthetase
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SpeciesZea mays
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byZmGln1-3 cDNA was provided by Dr. Bertrand Hirel, INRAe Versailles, France
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBIOS1458 was a gift from Christophe Sallaud (Addgene plasmid # 171715 ; http://n2t.net/addgene:171715 ; RRID:Addgene_171715) -
For your References section:
Impacts of environmental conditions, and allelic variation of cytosolic glutamine synthetase on maize hybrid kernel production. Amiour N, Decousset L, Rouster J, Quenard N, Buet C, Dubreuil P, Quillere I, Brule L, Cukier C, Dinant S, Sallaud C, Dubois F, Limami AM, Lea PJ, Hirel B. Commun Biol. 2021 Sep 17;4(1):1095. doi: 10.1038/s42003-021-02598-w. 10.1038/s42003-021-02598-w PubMed 34535763