pBbB8k-csg-nb112
(Plasmid
#171702)
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PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171702 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBbB8k
- Backbone size w/o insert (bp) 3763
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namecsgBACEFG, with nanobody fused to CsgA
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBbB8k-csg-nb112 was a gift from Neel Joshi (Addgene plasmid # 171702 ; http://n2t.net/addgene:171702 ; RRID:Addgene_171702) -
For your References section:
Simple Affinity-Based Method for Concentrating Viruses from Wastewater Using Engineered Curli Fibers. Birnbaum DP, Vilardi KJ, Anderson CL, Pinto AJ, Joshi NS. ACS ES T Water. 2022 Nov 11;2(11):1836-1843. doi: 10.1021/acsestwater.1c00208. Epub 2021 Dec 29. 10.1021/acsestwater.1c00208 PubMed 36778666
Map uploaded by the depositor.
