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Addgene

pCMV-BEACON2
(Plasmid #171698)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171698 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BEACON2
  • Alt name
    pCMV-SV40NLS-hAPOBEC3A_W98Y/W104A/Y130F-XTEN-dLbCpf1(D832A/E1006A/D1125A)-SV40NLS-SGGS-UGI-SV40NLS-op
  • Species
    H. sapiens (human); S. pyogenes, Bacteriophage PBS2
  • Insert Size (bp)
    4674
  • Mutation
    hAPOBEC3A_W98Y/W104A/Y130F
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-BEACON2 was a gift from Jia Chen (Addgene plasmid # 171698 ; http://n2t.net/addgene:171698 ; RRID:Addgene_171698)

  • For your References section:

    Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response. Wang X, Ding C, Yu W, Wang Y, He S, Yang B, Xiong YC, Wei J, Li J, Liang J, Lu Z, Zhu W, Wu J, Zhou Z, Huang X, Liu Z, Yang L, Chen J. Cell Rep. 2020 Jun 2;31(9):107723. doi: 10.1016/j.celrep.2020.107723. 10.1016/j.celrep.2020.107723 PubMed 32492431
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