pCMV-BEACON1
(Plasmid
#171697)
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PurposeExpresses BEACON1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBEACON1
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Alt namepCMV-SV40NLS-hAPOBEC3A_W104A/Y132D-XTEN-dLbCpf1(D832A/E1006A/D1125A)-SV40NLS-SGGS-UGI-SV40NLS-op
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SpeciesH. sapiens (human); S. pyogenes, Bacteriophage PBS2
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Insert Size (bp)4674
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MutationhAPOBEC3A_W104A/Y132D
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-BEACON1 was a gift from Jia Chen (Addgene plasmid # 171697 ; http://n2t.net/addgene:171697 ; RRID:Addgene_171697) -
For your References section:
Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response. Wang X, Ding C, Yu W, Wang Y, He S, Yang B, Xiong YC, Wei J, Li J, Liang J, Lu Z, Zhu W, Wu J, Zhou Z, Huang X, Liu Z, Yang L, Chen J. Cell Rep. 2020 Jun 2;31(9):107723. doi: 10.1016/j.celrep.2020.107723. 10.1016/j.celrep.2020.107723 PubMed 32492431