Skip to main content
Addgene

pU6-SasgRNA scaffold-CMV-nSaCas9
(Plasmid #171695)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171695 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA scaffold-nSaCas9-NLS
  • Alt name
    sgRNA scaffold
  • Alt name
    nSaCas9-NLS
  • Species
    Other
  • Insert Size (bp)
    3219
  • Mutation
    D10A
  • Promoter U6 and CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-SasgRNA scaffold-CMV-nSaCas9 was a gift from Jia Chen (Addgene plasmid # 171695 ; http://n2t.net/addgene:171695 ; RRID:Addgene_171695)

  • For your References section:

    Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations. Wang L, Xue W, Zhang H, Gao R, Qiu H, Wei J, Zhou L, Lei YN, Wu X, Li X, Liu C, Wu J, Chen Q, Ma H, Huang X, Cai C, Zhang Y, Yang B, Yin H, Yang L, Chen J. Nat Cell Biol. 2021 May 10. pii: 10.1038/s41556-021-00671-4. doi: 10.1038/s41556-021-00671-4. 10.1038/s41556-021-00671-4 PubMed 33972728
Related items:
Commonly requested with: