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pU6-ccdB-boxB-tBE-V5-mA3
(Plasmid #171693)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171693 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1a
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
  • Alt name
    ccdB-sgRNA scaffold-boxB
  • Alt name
    MCP-XTEN-NLS-UGI-mA3CDA1-TEV site-mA3CDA2-NLS-2A-N22p-XTEN-TEVc-NLS-2A-TEVn-NLS
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3108
  • Promoter U6 and EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The tBE system with gRNA could be constructed by linearing pU6-ccdB-boxB-tBE-V5-mA3 (backbone vector of tBE system) and inserting the gRNA sequences amplificating from pUC57-sgRNA-MS2-U6. The detailed protocol could be found in Supplementary Protocol in the publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-ccdB-boxB-tBE-V5-mA3 was a gift from Jia Chen (Addgene plasmid # 171693 ; http://n2t.net/addgene:171693 ; RRID:Addgene_171693)

  • For your References section:

    Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations. Wang L, Xue W, Zhang H, Gao R, Qiu H, Wei J, Zhou L, Lei YN, Wu X, Li X, Liu C, Wu J, Chen Q, Ma H, Huang X, Cai C, Zhang Y, Yang B, Yin H, Yang L, Chen J. Nat Cell Biol. 2021 May 10. pii: 10.1038/s41556-021-00671-4. doi: 10.1038/s41556-021-00671-4. 10.1038/s41556-021-00671-4 PubMed 33972728
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