pBHM2403
(Plasmid #171687)

• Purpose: pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.
• Depositing Lab: Hiten Madhani
• Publication: Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi: 10.1093/genetics/iyab180.

Backbone

• Vector backbone: pRS316
• Backbone size w/o insert (bp): 4839
• Total vector size (bp): 11183
• Vector type: Bacterial Expression, Yeast Expression, CRISPR
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CnoCAS9
• Alt name: Cryptococcus neoformans codon optimized S. pyogenes CAS9
• Insert Size (bp): 6344
• Promoter: TEF1

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GTAAAACGACGGCCAGT
• 3′ sequencing primer: CAGGAAACAGCTATGAC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry