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Addgene

pBHM2329
(Plasmid #171686)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171686 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4866
  • Total vector size (bp) 5222
  • Vector type
    Bacterial Expression, Yeast Expression, CRISPR
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCnU6-sgRNA_scaffold
  • Insert Size (bp)
    356
  • Promoter C. neoformans U6

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAGT
  • 3′ sequencing primer GTCATAGCTGTTTCCTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBHM2329 was a gift from Hiten Madhani (Addgene plasmid # 171686 ; http://n2t.net/addgene:171686 ; RRID:Addgene_171686)

  • For your References section:

    Short homology-directed repair using optimized Cas9 in the pathogen Cryptococcus neoformans enables rapid gene deletion and tagging. Huang MY, Joshi MB, Boucher MJ, Lee S, Loza LC, Gaylord EA, Doering TL, Madhani HD. Genetics. 2021 Oct 23. pii: 6409193. doi: 10.1093/genetics/iyab180. 10.1093/genetics/iyab180 PubMed 34791226
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