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Addgene

QUAS-0-T7-TetO-GFP_ColE1
(Plasmid #171662)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171662 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    T7-4-QUAS-GFP_ColE1
  • Backbone size w/o insert (bp) 1910
  • Total vector size (bp) 2847
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    QUAS-T7-TetO-GFP ssrA-T7 Stop
  • Insert Size (bp)
    938
  • Promoter T7
  • Tag / Fusion Protein
    • ssrA degradation tag (DAS+4) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer GAATAGTGTATGCGGCGACC
  • 3′ sequencing primer GGGGCGGAGCCTATGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    QUAS-0-T7-TetO-GFP_ColE1 was a gift from Tara Deans (Addgene plasmid # 171662 ; http://n2t.net/addgene:171662 ; RRID:Addgene_171662)

  • For your References section:

    Enhanced regulation of prokaryotic gene expression by a eukaryotic transcriptional activator. MacDonald IC, Seamons TR, Emmons JC, Javdan SB, Deans TL. Nat Commun. 2021 Jul 5;12(1):4109. doi: 10.1038/s41467-021-24434-9. 10.1038/s41467-021-24434-9 PubMed 34226549
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