BLING 1
(Plasmid
#171647)
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PurposeExpresses bioluminescent glutamate indicator in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBLING 1
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BLING 1 was a gift from Assaf Gilad (Addgene plasmid # 171647 ; http://n2t.net/addgene:171647 ; RRID:Addgene_171647) -
For your References section:
Bioluminescent Genetically Encoded Glutamate Indicators for Molecular Imaging of Neuronal Activity. Petersen ED, Lapan AP, Castellanos Franco EA, Fillion AJ, Crespo EL, Lambert GG, Grady CJ, Zanca AT, Orcutt R, Hochgeschwender U, Shaner NC, Gilad AA. ACS Synth Biol. 2023 Jul 14. doi: 10.1021/acssynbio.2c00687. 10.1021/acssynbio.2c00687 PubMed 37450884
