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Lmajor_gp63_10_0460_P460G_D463N_S465A
(Plasmid #171645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/Hygro(+)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LmjF.10.0460 P460G D463N S465A
  • Species
    L. major
  • Mutation
    P460G; D463N; S465A
  • Tag / Fusion Protein
    • Myc-His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence encoding the endogenous secretion signal peptide was removed and replaced with a Kozak consensus, start codon, and secrecon signal peptide sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lmajor_gp63_10_0460_P460G_D463N_S465A was a gift from Dennis Ko (Addgene plasmid # 171645 ; http://n2t.net/addgene:171645 ; RRID:Addgene_171645)

  • For your References section:

    Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factor. Antonia AL, Barnes AB, Martin AT, Wang L, Ko DC. PLoS Negl Trop Dis. 2021 Oct 28;15(10):e0009224. doi: 10.1371/journal.pntd.0009224. eCollection 2021 Oct. 10.1371/journal.pntd.0009224 PubMed 34710089
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