Lmajor_gp63_10_0460_E265A
(Plasmid #171641)

• Purpose: Expression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cells
• Depositing Lab: Dennis Ko
• Publication: Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;9:280. doi: 10.3389/fcimb.2019.00280. eCollection 2019.

Backbone

• Vector backbone: pcDNA3.1/Hygro(+)
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LmjF.10.0460 E265A
• Species: L. major
• Mutation: E265A
• Tag / Fusion Protein:
  • Myc-His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence encoding the endogenous secretion signal peptide was removed and replaced with a Kozak consensus, start codon, and secrecon signal peptide sequence.