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Addgene

CBM9-ID-F
(Plasmid #171639)

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Item Catalog # Description Quantity Price (USD)
Plasmid 171639 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pRSET5A
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 3700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    T7 Express lysY/Iq, NEB #C3013I
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Spike protein
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    791
  • GenBank ID
    NC_045512.2 MZ322554
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tag / Fusion Protein
    • CBM-9 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Esp3I (destroyed during cloning)
  • 3′ cloning site Esp3I (destroyed during cloning)
  • 5′ sequencing primer AAGGTCAGCGTGTTGGTATC or nFseq5A GATCTCGATCCCGCGAAATTAATAC
  • 3′ sequencing primer nRseq5A AAACCCCTCAAGACCCG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBM9-ID-F was a gift from Francis Nano (Addgene plasmid # 171639 ; http://n2t.net/addgene:171639 ; RRID:Addgene_171639)

  • For your References section:

    Escherichia coli recombinant expression of SARS-CoV-2 protein fragments. McGuire BE, Mela JE, Thompson VC, Cucksey LR, Stevens CE, McWhinnie RL, Winkler DFH, Pelech S, Nano FE. Microb Cell Fact. 2022 Feb 5;21(1):21. doi: 10.1186/s12934-022-01753-0. 10.1186/s12934-022-01753-0 PubMed 35123472
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