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Addgene

pCMV-AsCas12f1
(Plasmid #171614)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171614 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AsCas12f1 and sgRNA_v1
  • Alt name
    Cas12U3
  • Species
    Other

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-AsCas12f1 was a gift from Quanjiang Ji (Addgene plasmid # 171614 ; http://n2t.net/addgene:171614 ; RRID:Addgene_171614)

  • For your References section:

    Programmed genome editing by a miniature CRISPR-Cas12f nuclease. Wu Z, Zhang Y, Yu H, Pan D, Wang Y, Wang Y, Li F, Liu C, Nan H, Chen W, Ji Q. Nat Chem Biol. 2021 Sep 2. pii: 10.1038/s41589-021-00868-6. doi: 10.1038/s41589-021-00868-6. 10.1038/s41589-021-00868-6 PubMed 34475565
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