pET28a-SUMO-AsCas12f
(Plasmid
#171612)
-
PurposeAsCas12f1 heterologous expression plasmid
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 171612 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneColE1
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameAsCas12f1
-
Alt nameCas12U3
-
SpeciesOther
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that a Q114K mutation was identified in the SUMO tag. This mutation has no effect on the function of the plasmid, as the SUMO tag will be removed during the protein purification procedure.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET28a-SUMO-AsCas12f was a gift from Quanjiang Ji (Addgene plasmid # 171612 ; http://n2t.net/addgene:171612 ; RRID:Addgene_171612) -
For your References section:
Programmed genome editing by a miniature CRISPR-Cas12f nuclease. Wu Z, Zhang Y, Yu H, Pan D, Wang Y, Wang Y, Li F, Liu C, Nan H, Chen W, Ji Q. Nat Chem Biol. 2021 Sep 2. pii: 10.1038/s41589-021-00868-6. doi: 10.1038/s41589-021-00868-6. 10.1038/s41589-021-00868-6 PubMed 34475565