deltaLoop-MIDAS
(Plasmid
#171605)
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PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171605 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepETDuet-1
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5420
- Total vector size (bp) 6703
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Modifications to backboneAdded TEV site and SNAP tag.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
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SpeciesS. pombe (fission yeast)
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Insert Size (bp)915
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MutationReplaced amino acids 4458-4496 with ASGSGS linker
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Tags
/ Fusion Proteins
- His6 tag (N terminal on backbone)
- SNAP tag (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
deltaLoop-MIDAS was a gift from Tarun Kapoor (Addgene plasmid # 171605 ; http://n2t.net/addgene:171605 ; RRID:Addgene_171605) -
For your References section:
Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1. Mickolajczyk KJ, Olinares PDB, Niu Y, Chen N, Warrington SE, Sasaki Y, Walz T, Chait BT, Kapoor TM. Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18459-18469. doi: 10.1073/pnas.2002792117. Epub 2020 Jul 21. 10.1073/pnas.2002792117 PubMed 32694211
Map uploaded by the depositor.
