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Addgene

SNAP-MIDAS
(Plasmid #171604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 6802
  • Modifications to backbone
    Added TEV site and SNAP tag.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    1014
  • Tags / Fusion Proteins
    • His6 tag (N terminal on backbone)
    • SNAP tag (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SNAP-MIDAS was a gift from Tarun Kapoor (Addgene plasmid # 171604 ; http://n2t.net/addgene:171604 ; RRID:Addgene_171604)

  • For your References section:

    Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1. Mickolajczyk KJ, Olinares PDB, Niu Y, Chen N, Warrington SE, Sasaki Y, Walz T, Chait BT, Kapoor TM. Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18459-18469. doi: 10.1073/pnas.2002792117. Epub 2020 Jul 21. 10.1073/pnas.2002792117 PubMed 32694211
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