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pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3
(Plasmid #171588)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171588 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Probably higher yield with TB
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Bxb1 integrase

Gene/Insert 2

  • Gene/Insert name
    iCasp9

Gene/Insert 3

  • Gene/Insert name
    BFP

Gene/Insert 4

  • Gene/Insert name
    rtTA3G

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3 was a gift from Kenneth Matreyek (Addgene plasmid # 171588 ; http://n2t.net/addgene:171588 ; RRID:Addgene_171588)

  • For your References section:

    Mutants of human ACE2 differentially promote SARS-CoV and SARS-CoV-2 spike mediated infection. Shukla N, Roelle SM, Suzart VG, Bruchez AM, Matreyek KA. PLoS Pathog. 2021 Jul 16;17(7):e1009715. doi: 10.1371/journal.ppat.1009715. eCollection 2021 Jul. 10.1371/journal.ppat.1009715 PubMed 34270613
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