Skip to main content
Addgene

pET28-His-MBP-NLS-RfxCas13d-NLS-HA
(Plasmid #171586)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171586 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His-MBP-NLS-RfxCas13d-NLS-HA
  • Species
    Ruminococcus flavefaciens XPD3002
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.05.12.443920 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-His-MBP-NLS-RfxCas13d-NLS-HA was a gift from Neville Sanjana (Addgene plasmid # 171586 ; http://n2t.net/addgene:171586 ; RRID:Addgene_171586)

  • For your References section:

    Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells. Mendez-Mancilla A, Wessels HH, Legut M, Kadina A, Mabuchi M, Walker J, Robb GB, Holden K, Sanjana NE. Cell Chem Biol. 2022 Feb 17;29(2):321-327.e4. doi: 10.1016/j.chembiol.2021.07.011. Epub 2021 Aug 2. 10.1016/j.chembiol.2021.07.011 PubMed 34343484
Related items:
Commonly requested with: