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Addgene

-226bp goosecoid luciferase
(Plasmid #17158)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOLuc
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    goosecoid promoter
  • Alt name
    goosecoid
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    230
  • Entrez Gene
    gsc-a (a.k.a. Xgsc, goosecoid)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Generated by PCR using the 3' primer (nucleotides - 5 to
+ 12 relative to the transcription start site) and 5' primer to -226. The 3' primer contained a HindIII restriction site at its 5' terminus and the 5' primer contained a BamHI restriction site at its 5' terminus for directional cloning into
pOLuc.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    -226bp goosecoid luciferase was a gift from Ken Cho (Addgene plasmid # 17158 ; http://n2t.net/addgene:17158 ; RRID:Addgene_17158)

  • For your References section:

    Molecular mechanisms of Spemann's organizer formation: conserved growth factor synergy between Xenopus and mouse. Watabe T, Kim S, Candia A, Rothbacher U, Hashimoto C, Inoue K, Cho KW. Genes Dev. 1995 Dec 15. 9(24):3038-50. 10.1101/gad.9.24.3038 PubMed 8543150
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