M20 siamois-luciferase p01234
(Plasmid #17157)

• Depositing Lab: Randall Moon
• Publication: Brannon et al Genes Dev. 1997 Sep 15. 11(18):2359-70.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 4818
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: siamois promoter
• Species: X. laevis (frog)
• Insert Size (bp): 800
• Entrez Gene: sia1.L (a.k.a. XELAEV_18019203mg, Xsia, sia, sia1, siamois, siamois-A)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid History:
Constructed by David Kimelman, 6/6/96 XVII p. 98.

Notes for Use:
The -3.0kb-siamois promoter was directionally cloned into the KpnI and BglII sites of the luciferase reporter vector pGL3B (Promega) upstream of the luciferase ATG. The -0.8kb promoter construct was generated by digesting the -3.0kb/pGL3B construct with KpnI and at an internal EcoRI site to remove 2.2 kb of intervening sequence, filling in with DNA polymerase I large fragment and religating.

The Kpn site was lost but the R1 site was regenerated in the ligation. The size of the insert is approximate.

Citation: Brannon et al., Gene and Development 11:2359-2370, 1997.