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pMT-HGP-v3-NbVHH05-hIgG
(Plasmid #171565)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171565 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT-HGP-v3
  • Backbone manufacturer
    Ah-Ram Kim (Perrimon Lab)
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NbVHH05-human IgG-TEV-Avi-His
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBE2J1 (a.k.a. CGI-76, HSPC153, HSPC205, HSU93243, NCUBE-1, NCUBE1, UBC6, UBC6E, Ubc6p)
  • Tags / Fusion Proteins
    • BiP signal peptide (N terminal on insert)
    • hIgG1-hinge-N297A-TEV site/Avi-Tag/His-Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is extensively engineered vector. Originally, HGP (heat shock promoter-GFP-T2A-promoter) was added into pAW vector by Gibson cloning, resulting in pAW-HGP vector. And then, the cloning part including N-term Signal peptide / FLAG peptide and C-term TEV site/Avi-tag/His-tag) was introduced into pAW-HGP vector, resulting in pAW-HGP-v2. Finally, the Actin promoter was replaced with pMT promoter, resulting in final vector pMT-HGP-v3.

Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-HGP-v3-NbVHH05-hIgG was a gift from Norbert Perrimon & Hidde Ploegh (Addgene plasmid # 171565 ; http://n2t.net/addgene:171565 ; RRID:Addgene_171565)

  • For your References section:

    Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies. Xu J, Kim AR, Cheloha RW, Fischer FA, Li JSS, Feng Y, Stoneburner E, Binari R, Mohr SE, Zirin J, Ploegh HL, Perrimon N. Elife. 2022 Jan 25;11. pii: 74326. doi: 10.7554/eLife.74326. 10.7554/eLife.74326 PubMed 35076390
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