M19 pCS-twhh-dsl-1myc
(Plasmid #17156)

• Depositing Lab: Randall Moon
• Publication: Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4100

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dorsalin-1
• Alt name: dsl-1
• Species: G. gallus (chicken)
• Entrez Gene: GDF2 (a.k.a. DSL1)
• Tags / Fusion Proteins:
  • twhh promoter (D rerio) (N terminal on insert)
  • myc-tag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid History:

To place dsl-1myc after the twhh promoter, the DNA insert of dsl-1myc was first subcloned into the EcoRI site of expression vector pCS2+, which has a cytomegalovirus promoter and a polyadenylation site. The resulting plasmids were named pCS2+-dsl-1myc. To link dsl-1myc to the twhh promoter, the dsl-1myc insert was released from pCS2+-dsl-1myc by BamHI and XhoI digestion and then subcloned into pCS-twhh-beta -gal-vec BamHI and XhoI sites by replacing the beta -gal sequence. The final construct, pCS-twhh-dsl-1myc, contains the 5.2-kb twhh promoter and dsl-1myc.

Notes for Use:
Target dorsalin-1 expression in zebrafish notochord.

Citation: Du et al., 1997, J. Cell Biology.