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Addgene

p3xFLAG-CMV-fly Dicer
(Plasmid #171559)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFLAG-CMV 7.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dcr-1(CG4792)
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Dcr-1 (a.k.a. Dmel_CG4792, CG4792, DCR-1, DCR1, DICER, DICER-1, Dcr, Dcr1, Dicer, Dicer1, Dmel\CG4792, cg4792, dcr 1, dcr-1, dcr1, dicer, dicer-1, dicer1, dmDcr1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there are several polymorphisms compared to NCBI reference NP_524453.1- P164L, F399S, A702S, S796C, A1332V, P1338A, T1339I and L1345I. Depositor confirms these do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-CMV-fly Dicer was a gift from Yan Zeng (Addgene plasmid # 171559 ; http://n2t.net/addgene:171559 ; RRID:Addgene_171559)
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