pCMV-luc-miR-1
(Plasmid
#171556)
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Purposea positive control reporter with 3' UTR sequences perfectly complementary to miR-1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171556 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-luc
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namea sequences perfectly complementary to miR-1
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-luc-miR-1 was a gift from Yan Zeng (Addgene plasmid # 171556 ; http://n2t.net/addgene:171556 ; RRID:Addgene_171556) -
For your References section:
Differential Inhibition of Target Gene Expression by Human microRNAs. Li P, Chen Y, Juma CA, Yang C, Huang J, Zhang X, Zeng Y. Cells. 2019 Jul 30;8(8). pii: cells8080791. doi: 10.3390/cells8080791. 10.3390/cells8080791 PubMed 31366019