pGEX-4T-1-Rnt1-dsRBD(+6A)
(Plasmid
#171552)
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PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRnt1
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SpeciesS. cerevisiae (budding yeast)
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Mutationa six-alanine insertion in the L1 region
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Entrez GeneRNT1 (a.k.a. YMR239C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T-1-Rnt1-dsRBD(+6A) was a gift from Yan Zeng (Addgene plasmid # 171552 ; http://n2t.net/addgene:171552 ; RRID:Addgene_171552) -
For your References section:
Extending the L1 region in canonical double-stranded RNA-binding domains impairs their functions. Zhang X, Li P, Zhong H, Yang F, Liu F, Yedid G, Zeng Y. Acta Biochim Biophys Sin (Shanghai). 2021 Mar 26;53(4):463-471. doi: 10.1093/abbs/gmab014. 10.1093/abbs/gmab014 PubMed 33751023