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Addgene

pGEX-4T-1-Dicer-dsRBD (+6)
(Plasmid #171544)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171544 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DICER1
  • Species
    H. sapiens (human)
  • Mutation
    a six amino acid_TRLTEG_insertion in the L1 region
  • Entrez Gene
    DICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1-Dicer-dsRBD (+6) was a gift from Yan Zeng (Addgene plasmid # 171544 ; http://n2t.net/addgene:171544 ; RRID:Addgene_171544)

  • For your References section:

    Extending the L1 region in canonical double-stranded RNA-binding domains impairs their functions. Zhang X, Li P, Zhong H, Yang F, Liu F, Yedid G, Zeng Y. Acta Biochim Biophys Sin (Shanghai). 2021 Mar 26;53(4):463-471. doi: 10.1093/abbs/gmab014. 10.1093/abbs/gmab014 PubMed 33751023
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