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Addgene

p3xFLAG-zDrosha
(Plasmid #171540)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171540 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME18S-FL3-3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    drosha
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    rnasen (a.k.a. im:7150667, zgc:158612)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-zDrosha was a gift from Yan Zeng (Addgene plasmid # 171540 ; http://n2t.net/addgene:171540 ; RRID:Addgene_171540)

  • For your References section:

    Cloning, expression, and characterization of the zebrafish Dicer and Drosha enzymes. Li P, Tian Q, Hu M, Li W, Zhang X, Zeng Y. Biochem Biophys Res Commun. 2019 Jun 18;514(1):200-204. doi: 10.1016/j.bbrc.2019.04.122. Epub 2019 Apr 24. 10.1016/j.bbrc.2019.04.122 PubMed 31029426
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