M17 ptwhh-IGFP65C
(Plasmid
#17154)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17154 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametwhh promoter
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Alt nametiggy-winkle hedgehog
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)5200
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Entrez Geneshhb (a.k.a. twh, twhh)
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Tag
/ Fusion Protein
- b-globin intron, GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert: twhh promoter (5.2 Kb), rabbit Beta-globin intron 2 (IVS2, 640 bp), GFP (65C, 1Kb)
Plasmid History:
Source: zebrafish.
Notes for Use:
Gives both notochord and floor-plate specific expression of GFP, owing to having the intron present.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M17 ptwhh-IGFP65C was a gift from Randall Moon (Addgene plasmid # 17154 ; http://n2t.net/addgene:17154 ; RRID:Addgene_17154)